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Old 04-30-2019, 05:34 AM   #2
Senior Member
Location: New England

Join Date: Jun 2012
Posts: 195

One possibility that would explain this is that your phiX and library are not denaturing properly. How old is your NaOH? I always denature with NaOH, and then heat-denature my final combined library/phiX dilution.
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