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Old 04-15-2013, 06:46 AM   #1
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Location: Norway

Join Date: Apr 2013
Posts: 2
Default Differential gene expression on metatranscriptome data


I am new to RNA-seq analysis and wanted to ask for some advice. I'm working on several soil metagenomes taken at different temperatures. My plan is to map the reads from each sample against a reference genome and subsequently use DeSeq and Edger to screen for differentially expressed genes.
I was wondering if this will give reasonable results as I am only working with those metagenome reads that map to the reference. What do you think?

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