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Old 04-16-2013, 01:38 AM   #3
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Location: Norway

Join Date: Apr 2013
Posts: 2

Thank you for the reply and I will try to be a little more specific.

The experiment design included 3 biological replicates for different temperature points. It's biological soil samples but the coverage is unknown. Only thing I know is that the number of reads (Illumina) for each sample is the same. I have a reference that is either included in the sample or has multiple very closely related strains in it.

By the way: sorry for asking real basic questions. Just started with transcriptomics and didn't think about de novo assembly, yet. Could you hint me to some tools that map de novo assembled transcripts to a reference genome?

Thanke you very much,

Last edited by Tka; 04-16-2013 at 01:45 AM.
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