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Old 12-12-2013, 11:39 PM   #1
Location: Milano, Italy

Join Date: Aug 2011
Posts: 93
Default Adaptor dimer contamination

Dear All,

We are facing some problems with adaptor dimers in Illumina RNA seq library prep.

There is a small amount of adaptor dimer (at ~150 bp) in a few of the samples. We tried a second bead clean up on half of the sample, but lost a significant amount of material. We talked with Illumina tech support, and this is a tiny enough amount of adaptor that they felt if was fine to go forward. There will be some adaptor only reads that the analyst can filter from the data, but it should be a small percentage.

I attach the profile of the sample most contaminated, do you think this wont influence the amplicfication of my library??

Many thanks,
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