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Old 02-25-2010, 08:42 AM   #1
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Location: London, UK

Join Date: Jan 2010
Posts: 9
Default illumina alternative v1.5 protocol for small rna seq vs. the standard protocol

Hi All,
I was just wandering whether anybody had successfully used the illumina alternative v1.5 small RNA sample prep protocol . We tried but the only thing we could detect was adapter contamination.
If anybody has used it successfully can they comment on the 3' adapter ligation protocol ( the one using T4 RNA ligase 2 truncated). I was wandering why the portocol uses such a high Mg concentration ( 10mM I believe) and does not use PEG.
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