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Old 03-25-2010, 03:24 PM   #2
Bioo Scientific
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Location: Austin, Tx

Join Date: Oct 2009
Posts: 99
Default Troubleshooting v1.5 sample prep protocol

Where did you get your adapter? What concentration of adapter did you use and how many units of ligase did you use? Adding too much adapter may lead to primer dimers and other artificial PCR products.

We have used the v1.5 sample prep protocol using our own enzyme, AIR Ligase, a truncated T4 RNA Ligase 2 successfully with our adenylated adapters. We would be happy to send you our recommended protocol and a sample of T4 RNA Ligase 2 (truncated).

Dawn Obermoeller
Bioo Scientific

Last edited by Bioo Scientific; 09-15-2015 at 11:49 AM.
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