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Old 04-21-2015, 01:42 PM   #1
Location: Saint Louis, MO

Join Date: Apr 2015
Posts: 12
Default Adapting Illumina's 16s library prep protocol to function with 18s rRNA

I'm interested in studying eukaryotic parasite diversity in fecal samples that I've collected. There are a variety of Illumina myseq protocols out there, and of most interest to me is the one published by Illumina (16S metagenomic library preparation).

It seems like this protocol is cost effective and efficient, and I can't see why it would not work for 18s. My questions are...

1. For anyone that has attempted this protocol, are there particular steps to be weary of?

2. Is there any apparent reason why it would not work for 18s?

Thanks for any help.
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