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Old 10-08-2015, 08:00 AM   #3
Location: Heraklion, Greece

Join Date: Jan 2014
Posts: 79

Originally Posted by kerplunk412 View Post
Your plan sounds perfect. You can probably find a Kapa library prep protocol on their website, so you can use the PCR conditions from that. I think they are something like 98 degree melt and 65 degree anneal. Also, you should be checking the concentration of your PCR-free libraries by QPCR, so the primers from that kit should work as well.

Thanks. But the primers aren't supplied separately in the qPCR kit we use.

I realized the KAPA documentation specifies the primer sequences in the mix, and they are shorter than those above, especially Primer 1. I don't understand why the P5 primer above is so long. I'll try the KAPA primers and conditions.
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