SEQanswers - View Single Post - MiSeq v3 PE 300 run and Illumina's service

sajoshi · 04-04-2016, 08:51 AM

I think the libraries were prepared using Nextera. I did not make them, it was another user running on our machine. The run failed three time at the 300 cycle. I think he spiked 5%. He has had successful runs on MiSeq but using other kits and not this one. Illumina is troubleshooting the issue for me.

04-04-2016, 08:51 AM	#3
sajoshi Member Location: cleveland Join Date: Dec 2015 Posts: 26	I think the libraries were prepared using Nextera. I did not make them, it was another user running on our machine. The run failed three time at the 300 cycle. I think he spiked 5%. He has had successful runs on MiSeq but using other kits and not this one. Illumina is troubleshooting the issue for me.