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Old 04-04-2016, 08:51 AM   #3
sajoshi
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Location: cleveland

Join Date: Dec 2015
Posts: 26
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I think the libraries were prepared using Nextera. I did not make them, it was another user running on our machine. The run failed three time at the 300 cycle. I think he spiked 5%. He has had successful runs on MiSeq but using other kits and not this one. Illumina is troubleshooting the issue for me.
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