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Old 12-05-2016, 07:54 AM   #5
Senior Member
Location: CT

Join Date: Apr 2015
Posts: 243

Is this the same machine? Each machine clusters differently (I'd guess due to fluctuations in temperature control). FWIW, I cluster 16S runs at 6pM. When I go up to even 8pM I end up loosing so many clusters at PF that the output is roughly the same.
Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
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