SEQanswers - View Single Post - Terrible overclustering -- 16S EMP protocol

neko_33 · 12-05-2016, 08:46 AM

Dear thermophile,

Thank you very much for your suggestion. The machine we use is always the same.
But I will check if the temperature is decently controled.

In addition, it is surprising that even 8pM library is too high!

12-05-2016, 08:46 AM	#6
neko_33 Junior Member Location: Tokyo Join Date: Dec 2016 Posts: 3	Dear thermophile, Thank you very much for your suggestion. The machine we use is always the same. But I will check if the temperature is decently controled. In addition, it is surprising that even 8pM library is too high!