View Single Post
Old 12-05-2016, 10:54 AM   #7
Senior Member
Location: CT

Join Date: Apr 2015
Posts: 243

The EMP sequencing primers aren't the right TM (they're too low). I've had the opposite problem you've had in the past-regular libraries will sequence fine but I'll get very very few clusters on the 16s runs. This was on an older miseq and the temperature control module wasn't hitting and holding temps correctly. It was still within the error tolerance of illumina libraries but the emp libraries have a narrower tolerance.
Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
thermophile is offline   Reply With Quote