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Old 03-13-2018, 07:43 AM   #1
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Location: USA

Join Date: Mar 2018
Posts: 2
Default V4-V5 Region Using 2x250 - Enough Overlap?

Hi All,

We've been looking at the V4 region (515/806) in environmental samples for some time successfully. We'd like to move to the V4-V5 region using the 515/926 primers. That would result in a larger amplicon (~411 not including primer/barcode sequences).

Our sequencing provider runs 2x250 regularly but 2x300 only sporadically so the cost would be significantly more unless we have lots of samples. Does anybody have experience using 2x250 with this region? Will there be enough overlap of the paired reads to assemble?

Thanks for any insight!
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