SEQanswers - View Single Post - Non-Standard bases from RTA

cement_head · 10-30-2018, 04:56 PM

Weird question, does Illumina's current basecaller (RTA) on HiSeq3000 call non-standard bases? In other words, can you get something other than AGCT in the sequence read?

10-30-2018, 04:56 PM	#1
cement_head Senior Member Location: Oxford, Ohio Join Date: Mar 2012 Posts: 253	Non-Standard bases from RTA Weird question, does Illumina's current basecaller (RTA) on HiSeq3000 call non-standard bases? In other words, can you get something other than AGCT in the sequence read?