Quote:
Originally Posted by cement_head
I'd have to agree with GenoMax; super-important to have a consultation with the sequencing center about the library composition and ask them what they recommend. You probably should have had 10% PhiX spike-in added. HiSeq are terrible at dynamic calibration - MiSeqs are better (to a point).
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I see. Next time I will consider PhiX spike-in. Thanks, cement_head!