SEQanswers - View Single Post - Illumina1.8 Paired-End Barcode Splitting?

swbarnes2 · 10-25-2011, 02:46 PM

I guess you are running your own custom barcodes somehow? Like they are embedded in the sequence at both ends?

But in theory, if the reads came from the same flowcell, and lane, and tile, and xy-coordinates, (which is all in the original name given by Illumina to the read)they should be the two ends of the same DNA fragment.

10-25-2011, 02:46 PM	#2
swbarnes2 Senior Member Location: San Diego Join Date: May 2008 Posts: 912	I guess you are running your own custom barcodes somehow? Like they are embedded in the sequence at both ends? But in theory, if the reads came from the same flowcell, and lane, and tile, and xy-coordinates, (which is all in the original name given by Illumina to the read)they should be the two ends of the same DNA fragment.