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Old 04-22-2013, 08:42 AM   #1
Gorbenzer
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Location: italy

Join Date: Apr 2012
Posts: 45
Default fungal ITS profiling primers and 500bp Ion Torrent amplicon sequencing

Hi everyone,
i'm going to try ITS profiling using the primers developed here ( http://www.plosone.org/article/info:...l.pone.0040863 ) but the amplicon is ~500bp (470bp plus bacrcode and adapter sequences).

We use an Ion Torrent sequencer and we are using 200bp kits (we'll try 400bp kits soon).

Anyone knows if it's possible to use 200bp sequencing kits to sequence the first ~200bp bases of longer amplicons?

Thanks for the help!
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