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Old 04-23-2013, 09:30 PM   #2
genseq
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Location: Russia

Join Date: Dec 2007
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Quote:
Originally Posted by Gorbenzer View Post
Hi everyone,
i'm going to try ITS profiling using the primers developed here ( http://www.plosone.org/article/info:...l.pone.0040863 ) but the amplicon is ~500bp (470bp plus bacrcode and adapter sequences).

We use an Ion Torrent sequencer and we are using 200bp kits (we'll try 400bp kits soon).

Anyone knows if it's possible to use 200bp sequencing kits to sequence the first ~200bp bases of longer amplicons?

Thanks for the help!
500 pb DNA not suitable for 200bp kits because the chips are very sensitive to the total charge of DNA molecules immobilized on the ionospheres.
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