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Old 04-24-2013, 01:08 AM   #4
Gorbenzer
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Location: italy

Join Date: Apr 2012
Posts: 45
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Thanks for the reply,
usually we design 200bp specific primers but this time we have to amplify the fungal ITS region that is around 400/450 bp so primers can't be designed inside the variable region.

On the Ion Torrent comunity we have been suggested to try to cut by sonication or by enzime digestion the 450bp product in 200bp fragments.
We don't have a sonicator so we will try to use the cutting enzyme provided with Ion Torrent kits; The biggest problem will be try to define the correct exposure time.
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