View Single Post
Old 01-29-2014, 08:03 AM   #2
Location: USA

Join Date: Apr 2010
Posts: 76

Looks like RNA fragmentation or DNA contamination to me. The peaks are well correlated with the gel. If it was the chip, you'd have seen it across all samples. You might want to run an agarose gel yourself to confirm fragmentation. Maybe the DNAse treatment step was not very efficient? Or you didn't completely inactivate RNAse...

Last edited by flobpf; 01-29-2014 at 08:04 AM. Reason: grammar
flobpf is offline   Reply With Quote