View Single Post
Old 05-08-2015, 12:07 PM   #11
pmiguel
Senior Member
 
Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,317
Default

Quote:
Originally Posted by DNATECH View Post
Hi Miguel,

the input was 5ul of PhiX at 2 nM. So far we have used 2 nM concentrations for all our libraries/lanes. Illumina recommends up to 3 nM.
From what our FAS told us, I got the impression under-loading could be more detrimental than over-loading.
Wow, 2000 pM? I think the highest we ever went on the HiSeq2500 was 23 pM.

--
Phillip
pmiguel is offline   Reply With Quote