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Old 10-18-2016, 06:23 AM   #5
Location: Fargo

Join Date: Dec 2011
Posts: 66

If I ran dedupe, wouldn't that result in eliminating all duplicated kmers not just duplicated fragments? I want to assemble the resulting file, and I worry that normalizing the kmer counts to no greater than 1 would not be the best file for assembling. Am I wrong in this thinking? I toyed with the idea of just normalizing to 20 (which I intend to do at the end anyways, but figured that might leave cases where I still have more duplicate sequences than necessary.
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