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Old 02-14-2019, 01:52 PM   #2
cmbetts
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Location: Bay Area

Join Date: Jun 2012
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Very doable, although random priming isn't necessary for second strand if you're using standard Gubler-Hoffman, the RNaseH handles the priming. This Nature Methods paper from the Broad should give some inspiration on how to do it https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4712044/
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