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Old 03-08-2016, 01:11 AM   #42
Runuply
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Location: Japan

Join Date: Feb 2016
Posts: 6
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Quote:
Originally Posted by mantis View Post
Hi Runuply,

Yes, I did a 1.6V ampure treatment: The small primers won't bind to the peaks with this DNA/beads ratio.

Good luck!

Thanks Mantis.
I did the AMpure , it looks good.
By the way, may I know that, normally how much DNA can you get after lysis if you use 50K cells? Do you check the concentration before PCR amplification.
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