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Old 12-12-2016, 08:31 AM   #59
jamesdinn
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Location: Copenhagen

Join Date: Dec 2016
Posts: 2
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Quote:
Originally Posted by Runuply View Post
Thanks Mantis.
I did the AMpure , it looks good.
By the way, may I know that, normally how much DNA can you get after lysis if you use 50K cells? Do you check the concentration before PCR amplification.
Hi Runuply & Mantis,
I'm new user for ATAC-seq experiment, i see that you use AMpure beads to clean DNA, could i use it for the each purification step (after 30 min tagmentation, after PCR cycles...?) or only before loading in Bioanalyser?

thank you
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