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Old 08-11-2019, 08:36 AM   #2
Bukowski
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Location: Aberdeen, Scotland

Join Date: Jan 2010
Posts: 388
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Q1: Watch this: https://www.youtube.com/watch?v=fCd6B5HRaZ8

Q2: There is no requirement for your probes to be dsDNA. Why are you so interested in capturing both strands? They have exactly the same sequence right?

RNA-DNA is more stable "because thermodynamics". Im sure there is plenty of literature on the subject: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC342109/ And plenty more references here: https://www.quora.com/Why-is-the-RNA...NA-DNA-pairing

Last edited by Bukowski; 08-11-2019 at 08:39 AM.
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