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Old 08-12-2019, 12:08 PM   #4
Bukowski
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Location: Aberdeen, Scotland

Join Date: Jan 2010
Posts: 388
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Duplex sequencing is obviously better, but requires the use of MIDs. Without MIDs there is no way to assign which half of the duplex you're sequencing with a standard library prep and Illumina sequencing. This is why I linked the Illumina video, this is inherent in the method.

I assumed R loops were formed with mature RNA transcripts. The RNA probe lengths in a target capture might be too short for this to take place, but that's just a guess. Don't know, not an expert.
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