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Old 05-06-2019, 05:58 PM   #9
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Originally Posted by Adem80 View Post
But I mean, the 3' end of the nucleotide in the gap should be ligated to the phosphorylated 5' end of the oligo, I believe that's the reason why it should be phosphorylated, isn't it?
Can polymerases ligate?
I am just speculating. AFAIU, the remaining oligos will be kicked off by nick-translation/strand-displacement in addition to the gap filling; I assume there is no ligation happening. (Perhaps the phosphate group is required for the transposase loading instead?)

Last edited by luc; 05-06-2019 at 06:01 PM.
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