Thread: Tile is missing
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Old 08-04-2019, 01:00 PM   #3
JerryEckel
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Location: London, UK

Join Date: Aug 2019
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Quote:
Originally Posted by kmcarr View Post
It looks like you got hold of a very old (in Illumina time scale) FastQ file. Have a look at the https://www.weareteacherfinder.com/b...dent-problems/ discussion on the FastQ format Wikipedia page. The sequence headers in your file are the pre-CASAVA 1.8 format. The software you are trying to use (fastq_illumina_reads) is expecting the header format to be the post-CASAVA 1.8 format. I can't remember when that format version was first introduced but it was years ago.

What software package is the fastq_illumina_reads program from? Does it have a command line option to switch between the old and new FastQ sequence ID line formats?
Thanks for the info. I tried different ways, but they didn't work. That is the reason I started to look for the new information. And found this.
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