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Old 08-15-2019, 05:13 PM   #2
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Location: US

Join Date: Dec 2010
Posts: 421

It is not necessary to bring all libraries to the same concentration before pooling - although many labs do it that way.
You could also pool equal amounts of each library (e.g. same number of femtomoles).

In any case, you can always concentrate the pool with a bead-cleanup to reach the number requested by your facility (10-20nM seems a very high request, though).
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