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Old 04-19-2018, 03:20 PM   #4
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,187
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I should correct that by fragment numbers I meant fragments that are flanked by both RE and are in size selection window. With a 4x6 cutter in a 30 Gb genome I guess there will a lot more restriction fragments than 100k. Thanks for the reference as well.

Assuming bisulfite conversion will be after adapter ligation I wonder how you go around high cost of methylated adapters.
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