Does anyone know how to do use MIRA with a barcoding check prior to assembly???
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It asks for the trace files?
Hello,
It asks for the trace files (flowgrams), which you can omit with the command
mira --project=... --job=... --notraceinfo ...other parameters... according to Bastien Chevreux
but are the flowgrams useful? Because I want to directly compare MIRA to Newbler...Or are the qual files enough?
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Originally posted by raw937 View PostHello,
It asks for the trace files (flowgrams), which you can omit with the command
mira --project=... --job=... --notraceinfo ...other parameters... according to Bastien Chevreux
but are the flowgrams useful? Because I want to directly compare MIRA to Newbler...Or are the qual files enough?
See http://mira-assembler.sourceforge.ne..._xml_traceinfo
As maubp has already mentioned, you need to split your SFF files according to the MIDs used before you can use MIRA (.e.g. using Roche's sfftools).
Then you can either use Roche's sfftools or sff_extract to generate MIRA compliant FASTA input files.
hth, Sven
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