View Single Post
Old 10-30-2012, 06:59 AM   #4
Senior Member
Location: London

Join Date: Jun 2009
Posts: 298

I can't see how Nextera would work on amplicons of that size. You will need at least two insertion events in each amplicon to be able to generate library (as you need the adapters on both 3' and 5' ends). Plus you will lose around 50bp of the terminal ends due to non-transposition in those regions. I just think it's highly unlikely you could get anything at the end.
TonyBrooks is offline   Reply With Quote