View Single Post
Old 10-31-2012, 05:32 AM   #6
barak yaacov
Junior Member
 
Location: Israel

Join Date: Oct 2012
Posts: 2
Default

Thank you for all for your responses
There are two Nextera kits: The Nextera XT should work on amplicons up to 1000bp while the Nextera kit work on amplicons up to 5000bp.
I did the library prep today again and after PCR clean up I run the sample on bioanalyzer and thy look good pick about ~450 bp so the 0.6x AMPure work. I think I will skip the normalization step of the protocol and do it manually. What is the final concentration I can upload on the cartage of the miseq? If I have difference between the concentrations majored on Qubit and the one from the bioanalyzer to which one should I regard?
Many thanks
Barak
barak yaacov is offline   Reply With Quote