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Old 01-15-2015, 06:22 AM   #23
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Location: Oklahoma

Join Date: Sep 2009
Posts: 411

Remembering back to the HiseqX announcement the little holes that now hold the clustering oligos (which is why this is a "patterned" flowcell) allow 1 oligo to overpower any others that try to cluster in there. I suppose you could somehow get it to overcluster but it wouldn't be the same kind of overclustering we are used to.

Clustering still appears to be on the cBot.

Here's some back-of-excel calcs I did on prices posted for the new 3k/4k reagents. Throughput assumptions are made based on average numbers given by Illumina in their specs and 8-lane FCs.

Screen Shot 2015-01-15 at 9.19.09 AM.png
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