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Old 01-22-2015, 05:48 AM   #29
Location: Wageningen, Netherlands

Join Date: May 2009
Posts: 21

just visited an Illumina meeting in Brussels.

Current HiSeqs are not upgradable to the 3000 or 4000 versions. Hardware is too different between the systems.

4000 and 3000 can only sequence Human DNA with certain fragment length. WGS, exome and RNAseq will work well, other library types are not supported due to the limitations of the new exclusion amplification kinetics.

New NextSeq V2 chemistry was presented. New dyes in the V2 kits with much less spectral overlap compared to the V1 dyes. Discrimination between dyes is much better resulting in higher quality reads and lower error rates. Now comparable with their four dye chemistry.
All in all very informative again.
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