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Old 03-12-2011, 12:39 AM   #13
Junior Member
Location: UK

Join Date: Mar 2011
Posts: 4

Originally Posted by wildung View Post
Got 15Mb out of the 10Mb chip 1st time out of the box and cut a ridiculous number of corners in the protocol. From breaking emPCR to starting the seqn run can be an easy one person day. Still would like automation for library prep, emulsion breaking and bead purification, preferably yesterday. The 100Mb chip is now out,, ahead of schedule, and a Gb by Fall,, there seems to be plenty of head room for growth and the technology appears at first glance to be robust and not nearly as fussy as some had feared.
Sound like some promising data quality for a first real run Id be interested to hear more about which corners you cut in the protocols. Will you be posting on the Ion Community site too?
Fully agree Re: emulsion purifications. This is a key throughput and hands-on consideration in comparison to the MiSeq. BTW, there are rumours that a single unit automation solution for the PGM is imminent (next few weeks), but has anyone tried joining up a REM e to the PGM emulsions in the mean time?
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