Thread: ion torrent
View Single Post
Old 07-05-2011, 12:20 PM   #4
krobison
Senior Member
 
Location: Boston area

Join Date: Nov 2007
Posts: 747
Default

One possibility would be to design (and validate!) a series of long PCR amplicons to tile the gene (each one overlaps the previous one by a bit).

The pooled amplicons from each sample then generates a new fragment library. One thing to watch out for is that primer-derived sequences will have artificially high variant rates due to primer synthesis errors.
krobison is offline   Reply With Quote