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Old 11-18-2013, 06:37 AM   #1
Martin Mikkelsen
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Location: Denmark

Join Date: Jun 2010
Posts: 11
Default Problems getting results using Illumina / NEB kit for small RNA library prep.

Dear all,

Does anyone have any experience with these kits?

I was able to make one successful build using the Illumina kit and since then has everything failed. I have also tried the similar kit from NEB.

I have used Ambion brain, liver and testes including some internal in-house controls containing known amounts of small RNA species.

How stable are the reagents in the kit?
Should I worry about my samples? I have used the recommend 1 g input material for both kits. And QC-ed my samples using the BioAnalyzer RNA kit.

Hope that someone has an idea of what could be wrong or how to proceed!
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