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Old 06-10-2016, 07:07 AM   #1
sajoshi
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Location: cleveland

Join Date: Dec 2015
Posts: 26
Default TruSeq smallRNA libraries

I am doing a TruSeq smallRNA library prep. When I was doing Ligate 3' adapter, and came to adding the STP step, the tube in the kit was empty (Illumina glitch). Illumina said to keep samples at -80 and shipped me the STP. Then I continued. When I checked BioA traces (see attached) the band is not where it should be. So it did not work and I will have to repeat. Any suggestions/feedback if you have any experience with this library prep.
Attached Files
File Type: pdf June 9_O'tiernney-ginn_initial check_DNA HS.pdf (1.68 MB, 35 views)
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