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Old 06-17-2016, 07:17 AM   #5
sajoshi
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Location: cleveland

Join Date: Dec 2015
Posts: 26
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Quote:
Originally Posted by kerplunk412 View Post
The RNA looks good to me, but the libraries seem to be just adapter-dimer. How much RNA input did you use?

Also, I would like to mention that the TruSeq kit is about the worst Small RNA-Seq kit you can get, at least in terms of cost and data quality. I highly recommend you check out the NEXTflex Small RNA Sequencing Kit v3, as it uses randomized adapters to reduce ligase bias and therefore generate more accurate data and greater discovery/detection rates, it includes completely gel-free or low-input options, and it is much more cost effective.

As a disclaimer, I work for Bioo Scientific.
I just send you a message asking a question. Please let me know.
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