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Old 08-19-2016, 08:54 AM   #5
thermophile
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Location: CT

Join Date: Apr 2015
Posts: 243
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what do your fails look like? qscore heatmaps and % base

Since I had access to another MiSeq, I was able to run my problem library on that machine (something about that library just made the annealing temp issue worse). After replacing my TCM, the FAE matched my temps to the other machine on campus.
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