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Old 08-19-2016, 03:49 PM   #8
HESmith
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Location: Bethesda MD

Join Date: Oct 2009
Posts: 505
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If you're going to use a mixture of sequencing primers, why not extend the 5' side (per @kerplunk412) by adding the index sequences (plus upstream P5/P7 bases, if necessary to obtain Tm = 65)? Yes, it's 40 oligos, but those are cheap. And it has the advantage of sequencing all of the diversity that you want to capture.

Last edited by HESmith; 08-19-2016 at 03:54 PM.
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