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Old 09-20-2016, 11:00 AM   #8
kerplunk412
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Location: Bioo Scientific, Austin, TX, USA

Join Date: Jun 2012
Posts: 119
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TriLink has indeed modified the adapters to greatly reduce adapter-dimer formation, but I am not sure exactly how it works. In my experience their kit works well but still has big issues with bias.

A peak at ~60 bp is most likely excess PCR primer and will not interfere with clustering, as it will not be able to amplify on the flow cell.
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