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Old 09-21-2016, 07:33 AM   #10
kerplunk412
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Location: Bioo Scientific, Austin, TX, USA

Join Date: Jun 2012
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Unless there is a large amount of excess PCR primer compared to actual library product it should not occupy enough primers on the flow cell to make any difference. The flow cell has enough primers on it to allow single library molecules to make clusters of about 1,000 strands (according to Wikipedia) through bridge amplification, so since the excess PCR primer is not amplified the number of primer sites it occupies on the flow cell will be negligible.
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