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Old 11-21-2019, 11:49 PM   #1
ECorrales
Junior Member
 
Location: Germany

Join Date: Nov 2019
Posts: 3
Default How to pool ATAC-seq libraries in equimolar concentrations?

Hi! I have 24 ATAC libraries prepared for sequencing. For this, I want to pool them all together. I ran them on the TapeStation and I have the distinctive nucleosomal banding pattern (see attached files). I'm hesitating a bit on how to properly estimate the molarity of every library when I have multiple bands, since the Tape is not always detecting the same bands. Any suggestion about this? Should I just use Qubit quantification for pooling?
Attached Files
File Type: pdf 20191031_libraries1-9.pdf (594.7 KB, 15 views)
File Type: pdf 20191107_libraries10-24.pdf (860.9 KB, 15 views)
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