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Old 11-22-2019, 11:59 AM   #4
kmcarr
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Location: USA, Midwest

Join Date: May 2008
Posts: 1,177
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Quote:
Originally Posted by ECorrales View Post
Thank you for your reply. Would you say these peaks corresponds to the upper marker? I was assuming they were larger un-tagmented regions, so I was planning to do a second size selection round with Ampure beads to remove these peaks. But if these are the markers then a second size selection might be unnecessary.
What I am saying is that for those 5 lanes I mentioned there are 2 very closely spaced peaks, one labeled as a peak of ~1,260-1,270bp and the peak just after that labeled as the Upper marker. The software has incorrectly identified the later migrating peak as the Upper marker. In the TapeStation analysis software you should select the peak which is labeled ~1,270 and manually set that as the upper marker. If you do not correct the upper marker then the size calculation for these lanes will be incorrect.
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