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Old 09-03-2012, 01:00 PM   #6
el_Davido
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Location: Germany

Join Date: Sep 2012
Posts: 5
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For me as a layperson it seems that the number of reads does correspond to the signal intensity. Is that correct?

The file comes from a paper where they performed chromatin-immunoprecipitation for STAT5, followed by sequencing.

Is it a valid approach to count the number of individual reads?

Code:
chr1 3001356 3001381 U0 0 -
chr1 3001356 3001381 U0 0 -
chr1 3001356 3001381 U0 0 -
chr1 3001356 3001381 U0 0 -
chr1 3007329 3007354 U0 0 +
chr1 3013242 3013267 U0 0 -
chr1 3016493 3016518 U0 0 -
chr1 3016493 3016518 U0 0 -
chr1 3016493 3016518 U0 0 -
Would become (in my view)

Code:
chr1 3001356 3001381 4
chr1 3007329 3007354 1
chr1 3013242 3013267 1
chr1 3016493 3016518 3

Thanks for your support so far, SES!
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