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Old 12-14-2013, 12:56 PM   #2
Genohub
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Default Ampure Size Selection to remove adapter dimer

Hi Paolo,

What bead to sample volume ratio did you use for your cleanup? If you use the wrong ratio, loss of library is possible. See slide 52: http://www.broadinstitute.org/files/...PrepSlides.pdf

A ratio of 0.7-0.8x is pretty effective at removing the adapter dimer band without resulting in sample loss.

I would personally do one more cleanup to completely remove the dimer band. Try it out on a non-precious sample first to test.

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