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Old 02-01-2013, 04:37 AM   #1
Shishir
Member
 
Location: Germany

Join Date: Nov 2012
Posts: 22
Default How to parse FASTQ file?

I am new in NGS. I need to format some fastq files. For example I want to replace characters /1 and /2 with -1 and - 2 in the files having data like @HWUSI-EAS1671_0001:5:1:1022:10290/1.
I tried to do it in perl with pattern matching but the files are too big to parse. Could anyone please give me suggestion on share any code?
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